## htsjdk.samtools.metrics.StringHeader # MarkDuplicates --INPUT SRX7354318.sorted.bam --OUTPUT SRX7354318.markdup.sorted.bam --METRICS_FILE SRX7354318.markdup.sorted.MarkDuplicates.metrics.txt --REMOVE_DUPLICATES false --ASSUME_SORTED true --TMP_DIR tmp --VALIDATION_STRINGENCY LENIENT --REFERENCE_SEQUENCE Homo_sapiens_assembly38_noALT_noHLA_noDecoy_patched_ERCC92.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Oct 30 21:57:42 GMT 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 77160 52441709 10604707 3468956 68085 32061140 0 0.611566 22600887 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 11393586 11393586 2.0 1.09824 4710185 4710185 3.0 1.107891 1909072 1909072 4.0 1.10884 828456 828456 5.0 1.108933 413986 413986 6.0 1.108942 240795 240795 7.0 1.108943 156899 156899 8.0 1.108943 111732 111732 9.0 1.108943 83751 83751 10.0 1.108943 64738 64738 11.0 1.108943 51998 51998 12.0 1.108943 41824 41824 13.0 1.108943 35161 35161 14.0 1.108943 29391 29391 15.0 1.108943 25372 25372 16.0 1.108943 21533 21533 17.0 1.108943 18736 18736 18.0 1.108943 16590 16590 19.0 1.108943 14705 14705 20.0 1.108943 13149 13149 21.0 1.108943 11875 11875 22.0 1.108943 10643 10643 23.0 1.108943 9532 9532 24.0 1.108943 8659 8659 25.0 1.108943 7940 7940 26.0 1.108943 7372 7372 27.0 1.108943 6778 6778 28.0 1.108943 6097 6097 29.0 1.108943 5637 5637 30.0 1.108943 5123 5123 31.0 1.108943 4883 4883 32.0 1.108943 4522 4522 33.0 1.108943 4354 4354 34.0 1.108943 4006 4006 35.0 1.108943 3681 3681 36.0 1.108943 3541 3541 37.0 1.108943 3346 3346 38.0 1.108943 3116 3116 39.0 1.108943 2904 2904 40.0 1.108943 2839 2839 41.0 1.108943 2640 2640 42.0 1.108943 2553 2553 43.0 1.108943 2296 2296 44.0 1.108943 2283 2283 45.0 1.108943 2195 2195 46.0 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