## htsjdk.samtools.metrics.StringHeader # MarkDuplicates --INPUT SRX7354325.sorted.bam --OUTPUT SRX7354325.markdup.sorted.bam --METRICS_FILE SRX7354325.markdup.sorted.MarkDuplicates.metrics.txt --REMOVE_DUPLICATES false --ASSUME_SORTED true --TMP_DIR tmp --VALIDATION_STRINGENCY LENIENT --REFERENCE_SEQUENCE Homo_sapiens_assembly38_noALT_noHLA_noDecoy_patched_ERCC92.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false ## htsjdk.samtools.metrics.StringHeader # Started on: Tue Oct 31 00:58:22 GMT 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 64814 59794158 10191907 7798324 55623 41913033 0 0.70104 18633972 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 7294277 7294277 2.0 1.040402 4308601 4308601 3.0 1.042034 2423662 2423662 4.0 1.0421 1348390 1348390 5.0 1.042103 766916 766916 6.0 1.042103 453734 453734 7.0 1.042103 283241 283241 8.0 1.042103 186808 186808 9.0 1.042103 129870 129870 10.0 1.042103 95894 95894 11.0 1.042103 72589 72589 12.0 1.042103 57979 57979 13.0 1.042103 46799 46799 14.0 1.042103 39128 39128 15.0 1.042103 32821 32821 16.0 1.042103 27732 27732 17.0 1.042103 23907 23907 18.0 1.042103 21066 21066 19.0 1.042103 18425 18425 20.0 1.042103 16327 16327 21.0 1.042103 14645 14645 22.0 1.042103 12988 12988 23.0 1.042103 11657 11657 24.0 1.042103 10694 10694 25.0 1.042103 9595 9595 26.0 1.042103 8725 8725 27.0 1.042103 8198 8198 28.0 1.042103 7494 7494 29.0 1.042103 6788 6788 30.0 1.042103 6355 6355 31.0 1.042103 5751 5751 32.0 1.042103 5406 5406 33.0 1.042103 5046 5046 34.0 1.042103 4662 4662 35.0 1.042103 4334 4334 36.0 1.042103 4061 4061 37.0 1.042103 3919 3919 38.0 1.042103 3613 3613 39.0 1.042103 3461 3461 40.0 1.042103 3205 3205 41.0 1.042103 2944 2944 42.0 1.042103 2900 2900 43.0 1.042103 2675 2675 44.0 1.042103 2526 2526 45.0 1.042103 2404 2404 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