## htsjdk.samtools.metrics.StringHeader # MarkDuplicates --INPUT SRX7354384.sorted.bam --OUTPUT SRX7354384.markdup.sorted.bam --METRICS_FILE SRX7354384.markdup.sorted.MarkDuplicates.metrics.txt --REMOVE_DUPLICATES false --ASSUME_SORTED true --TMP_DIR tmp --VALIDATION_STRINGENCY LENIENT --REFERENCE_SEQUENCE Homo_sapiens_assembly38_noALT_noHLA_noDecoy_patched_ERCC92.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false ## htsjdk.samtools.metrics.StringHeader # Started on: Mon Oct 30 23:56:16 GMT 2023 ## METRICS CLASS picard.sam.DuplicationMetrics LIBRARY UNPAIRED_READS_EXAMINED READ_PAIRS_EXAMINED SECONDARY_OR_SUPPLEMENTARY_RDS UNMAPPED_READS UNPAIRED_READ_DUPLICATES READ_PAIR_DUPLICATES READ_PAIR_OPTICAL_DUPLICATES PERCENT_DUPLICATION ESTIMATED_LIBRARY_SIZE Unknown Library 74543 56069686 9843105 5289045 62352 32599765 0 0.581585 26763811 ## HISTOGRAM java.lang.Double BIN CoverageMult all_sets non_optical_sets 1.0 1 15370879 15370879 2.0 1.123073 4671777 4671777 3.0 1.138219 1498672 1498672 4.0 1.140084 585953 585953 5.0 1.140313 293983 293983 6.0 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1 1 1584.0 0 2 2 1587.0 0 1 1 1592.0 0 1 1 1601.0 0 1 1 1602.0 0 1 1 1620.0 0 1 1 1624.0 0 1 1 1630.0 0 1 1 1635.0 0 1 1 1636.0 0 1 1 1639.0 0 1 1 1640.0 0 1 1 1641.0 0 1 1 1658.0 0 1 1 1730.0 0 1 1 1734.0 0 1 1 1742.0 0 1 1 1745.0 0 1 1 1764.0 0 1 1 1777.0 0 1 1 1798.0 0 1 1 1801.0 0 1 1 1818.0 0 1 1 1831.0 0 1 1 1854.0 0 1 1 1861.0 0 1 1 1888.0 0 1 1 1902.0 0 1 1 1918.0 0 1 1 1960.0 0 2 2 1962.0 0 1 1 1996.0 0 1 1 2063.0 0 1 1 2093.0 0 1 1 2106.0 0 1 1 2138.0 0 1 1 2188.0 0 1 1 2191.0 0 1 1 2219.0 0 1 1 2274.0 0 1 1 2288.0 0 1 1 2319.0 0 1 1 2651.0 0 1 1 3038.0 0 1 1 3903.0 0 1 1